LOCUS       pSK-Moderna-Swt        6910 bp    DNA     circular SYN 18-FEB-2022
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 19, 2021 from SnapGene Viewer 5.1.7 to Vector
            NTI(R) format
            https://www.snapgene.com
COMMENT     Storage Type: Basic
            Original Author: 
            Demo User
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14264407|
COMMENT     VNTDBDATE|-14269622|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSK-Moderna-Swt|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    complement(263..569)
                     /vntifkey="21"
                     /label=f1
     primer          complement(752..786)
                     /vntifkey="27"
                     /label=MMrnd5T7
                     /note="sequence: ttctctcttatttccctatagtgagtcgtattacg"
     misc_feature    754..773
                     /vntifkey="21"
                     /label=T7\promoter
     primer          759..794
                     /vntifkey="27"
                     /label=P-MrndT75
                     /note="sequence: cgactcactatagggaaataagagagaaaagaagag"
     primer          772..794
                     /vntifkey="27"
                     /label=Mrdn\B1F-GG
                     /note="sequence: ggaaataagagagaaaagaagag"
     misc_feature    773..827
                     /vntifkey="21"
                     /label=5'\UTR
     CDS             828..4646
                     /vntifkey="4"
                     /label=S\Protein
     primer          2073..2093
                     /vntifkey="27"
                     /label=PMord1300
                     /note="sequence: ggcaagatcgccgactacaac"
     primer          2166..2198
                     /vntifkey="27"
                     /label=PMordL452
                     /note="sequence: ggcaactacaactacctgtaccggctgttccgg"
     primer          complement(2166..2198)
                     /vntifkey="27"
                     /label=MMordL452
                     /note="sequence: ccggaacagccggtacaggtagttgtagttgcc"
     misc_feature    2181..2183
                     /vntifkey="21"
                     /label=L\452
     primer          2262..2294
                     /vntifkey="27"
                     /label=PMordE484
                     /note="sequence: ccttgcaacggcgtggagggcttcaactgctac"
     primer          complement(2262..2294)
                     /vntifkey="27"
                     /label=MMordE484
                     /note="sequence: gtagcagttgaagccctccacgccgttgcaagg"
     misc_feature    2277..2279
                     /vntifkey="21"
                     /label=E\484
     primer          2313..2345
                     /vntifkey="27"
                     /label=PMordN501
                     /note="sequence: ggcttccagcccaccaacggcgtgggctaccag"
     primer          complement(2313..2345)
                     /vntifkey="27"
                     /label=MMordN501
                     /note="sequence: ctggtagcccacgccgttggtgggctggaagcc"
     misc_feature    2328..2330
                     /vntifkey="21"
                     /label=N\501
     primer          complement(2337..2348)
                     /vntifkey="27"
                     /label=MMordN501
                     /note="sequence: ctggtagcccacgccgttggtgggctggaagcc"
     primer          2652..2684
                     /vntifkey="27"
                     /label=PMordD614
                     /note="sequence: gccgtgctgtaccaggacgtgaactgcaccgag"
     primer          complement(2652..2684)
                     /vntifkey="27"
                     /label=MMordD614
                     /note="sequence: ctcggtgcagttcacgtcctggtacagcacggc"
     misc_feature    2667..2669
                     /vntifkey="21"
                     /label=D\614
     primer          complement(2793..2816)
                     /vntifkey="27"
                     /label=B1R2
                     /note="sequence: gtcgcactcgtagctgttgttcac"
     primer          2793..2816
                     /vntifkey="27"
                     /label=B2F2
                     /note="sequence: gtgaacaacagctacgagtgcgac"
     misc_feature    2796..2815
                     /vntifkey="21"
                     /label=B2F
     misc_feature    2796..2815
                     /vntifkey="21"
                     /label=B1R
     primer          2853..2885
                     /vntifkey="27"
                     /label=PMordP681
                     /note="sequence: acccagaccaattcaccccggagggcaaggagc"
     primer          complement(2853..2885)
                     /vntifkey="27"
                     /label=MMordP681
                     /note="sequence: gctccttgccctccggggtgaattggtctgggt"
     misc_feature    2868..2870
                     /vntifkey="21"
                     /label=P\681
     primer          3660..3692
                     /vntifkey="27"
                     /label=PMordD950
                     /note="sequence: ctgggcaagctgcaggacgtggtgaaccagaac"
     primer          complement(3660..3692)
                     /vntifkey="27"
                     /label=MMordD950
                     /note="sequence: gttctggttcaccacgtcctgcagcttgcccag"
     misc_feature    3675..3677
                     /vntifkey="21"
                     /label=D\950
     primer          complement(3788..3808)
                     /vntifkey="27"
                     /label=MMord3035
                     /note="sequence: tcgatctgcacctcggcctcg"
     misc_feature    4647..4655
                     /vntifkey="21"
                     /label=Stop
     misc_feature    4656..4765
                     /vntifkey="21"
                     /label=3'\UTR
     primer          complement(4849..4871)
                     /vntifkey="27"
                     /label=T3P
                     /note="sequence: cgcaattaaccctcactaaaggg"
     misc_feature    complement(4849..4868)
                     /vntifkey="21"
                     /label=T3\P
     misc_feature    complement(5235..5902)
                     /vntifkey="21"
                     /label=pUC\ori
     misc_feature    complement(6053..6910)
                     /vntifkey="21"
                     /label=Ampicillin
BASE COUNT     1628 a      2089 c      1855 g      1338 t 
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc 
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 
      721 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggaaataag 
      781 agagaaaaga agagtaagaa gaaatataag accccggcgc cgccaccatg ttcgtgttcc 
      841 tggtgctgct gcccctggtg agcagccagt gcgtgaacct gaccacccgg acccagctgc 
      901 caccagccta caccaacagc ttcacccggg gcgtctacta ccccgacaag gtgttccgga 
      961 gcagcgtcct gcacagcacc caggacctgt tcctgccctt cttcagcaac gtgacctggt 
     1021 tccacgccat ccacgtgagc ggcaccaacg gcaccaagcg gttcgacaac cccgtgctgc 
     1081 ccttcaacga cggcgtgtac ttcgccagca ccgagaagag caacatcatc cggggctgga 
     1141 tcttcggcac caccctggac agcaagaccc agagcctgct gatcgtgaat aacgccacca 
     1201 acgtggtgat caaggtgtgc gagttccagt tctgcaacga ccccttcctg ggcgtgtact 
     1261 accacaagaa caacaagagc tggatggaga gcgagttccg ggtgtacagc agcgccaaca 
     1321 actgcacctt cgagtacgtg agccagccct tcctgatgga cctggagggc aagcagggca 
     1381 acttcaagaa cctgcgggag ttcgtgttca agaacatcga cggctacttc aagatctaca 
     1441 gcaagcacac cccaatcaac ctggtgcggg atctgcccca gggcttctca gccctggagc 
     1501 ccctggtgga cctgcccatc ggcatcaaca tcacccggtt ccagaccctg ctggccctgc 
     1561 accggagcta cctgacccca ggcgacagca gcagcgggtg gacagcaggc gcggctgctt 
     1621 actacgtggg ctacctgcag ccccggacct tcctgctgaa gtacaacgag aacggcacca 
     1681 tcaccgacgc cgtggactgc gccctggacc ctctgagcga gaccaagtgc accctgaaga 
     1741 gcttcaccgt ggagaagggc atctaccaga ccagcaactt ccgggtgcag cccaccgaga 
     1801 gcatcgtgcg gttccccaac atcaccaacc tgtgcccctt cggcgaggtg ttcaacgcca 
     1861 cccggttcgc cagcgtgtac gcctggaacc ggaagcggat cagcaactgc gtggccgact 
     1921 acagcgtgct gtacaacagc gccagcttca gcaccttcaa gtgctacggc gtgagcccca 
     1981 ccaagctgaa cgacctgtgc ttcaccaacg tgtacgccga cagcttcgtg atccgtggcg 
     2041 acgaggtgcg gcagatcgca cccggccaga caggcaagat cgccgactac aactacaagc 
     2101 tgcccgacga cttcaccggc tgcgtgatcg cctggaacag caacaacctc gacagcaagg 
     2161 tgggcggcaa ctacaactac ctgtaccggc tgttccggaa gagcaacctg aagcccttcg 
     2221 agcgggacat cagcaccgag atctaccaag ccggctccac cccttgcaac ggcgtggagg 
     2281 gcttcaactg ctacttccct ctgcagagct acggcttcca gcccaccaac ggcgtgggct 
     2341 accagcccta ccgggtggtg gtgctgagct tcgagctgct gcacgcccca gccaccgtgt 
     2401 gtggccccaa gaagagcacc aacctggtga agaacaagtg cgtgaacttc aacttcaacg 
     2461 gccttaccgg caccggcgtg ctgaccgaga gcaacaagaa attcctgccc tttcagcagt 
     2521 tcggccggga catcgccgac accaccgacg ctgtgcggga tccccagacc ctggagatcc 
     2581 tggacatcac cccttgcagc ttcggcggcg tgagcgtgat caccccaggc accaacacca 
     2641 gcaaccaggt ggccgtgctg taccaggacg tgaactgcac cgaggtgccc gtggccatcc 
     2701 acgccgacca gctgacaccc acctggcggg tctacagcac cggcagcaac gtgttccaga 
     2761 cccgggccgg ttgcctgatc ggcgccgagc acgtgaacaa cagctacgag tgcgacatcc 
     2821 ccatcggcgc cggcatctgt gccagctacc agacccagac caattcaccc cggagggcaa 
     2881 ggagcgtggc cagccagagc atcatcgcct acaccatgag cctgggcgcc gagaacagcg 
     2941 tggcctacag caacaacagc atcgccatcc ccaccaactt caccatcagc gtgaccaccg 
     3001 agattctgcc cgtgagcatg accaagacca gcgtggactg caccatgtac atctgcggcg 
     3061 acagcaccga gtgcagcaac ctgctgctgc agtacggcag cttctgcacc cagctgaacc 
     3121 gggccctgac cggcatcgcc gtggagcagg acaagaacac ccaggaggtg ttcgcccagg 
     3181 tgaagcagat ctacaagacc cctcccatca aggacttcgg cggcttcaac ttcagccaga 
     3241 tcctgcccga ccccagcaag cccagcaagc ggagcttcat cgaggacctg ctgttcaaca 
     3301 aggtgaccct agccgacgcc ggcttcatca agcagtacgg cgactgcctc ggcgacatag 
     3361 ccgcccggga cctgatctgc gcccagaagt tcaacggcct gaccgtgctg cctcccctgc 
     3421 tgaccgacga gatgatcgcc cagtacacca gcgccctgtt agccggaacc atcaccagcg 
     3481 gctggacttt cggcgctgga gccgctctgc agatcccctt cgccatgcag atggcctacc 
     3541 ggttcaacgg catcggcgtg acccagaacg tgctgtacga gaaccagaag ctgatcgcca 
     3601 accagttcaa cagcgccatc ggcaagatcc aggacagcct gagcagcacc gctagcgccc 
     3661 tgggcaagct gcaggacgtg gtgaaccaga acgcccaggc cctgaacacc ctggtgaagc 
     3721 agctgagcag caacttcggc gccatcagca gcgtgctgaa cgacatcctg agccggctgg 
     3781 accctcccga ggccgaggtg cagatcgacc ggctgatcac tggccggctg cagagcctgc 
     3841 agacctacgt gacccagcag ctgatccggg ccgccgagat tcgggccagc gccaacctgg 
     3901 ccgccaccaa gatgagcgag tgcgtgctgg gccagagcaa gcgggtggac ttctgcggca 
     3961 agggctacca cctgatgagc tttccccaga gcgcacccca cggagtggtg ttcctgcacg 
     4021 tgacctacgt gcccgcccag gagaagaact tcaccaccgc cccagccatc tgccacgacg 
     4081 gcaaggccca ctttccccgg gagggcgtgt tcgtgagcaa cggcacccac tggttcgtga 
     4141 cccagcggaa cttctacgag ccccagatca tcaccaccga caacaccttc gtgagcggca 
     4201 actgcgacgt ggtgatcggc atcgtgaaca acaccgtgta cgatcccctg cagcccgagc 
     4261 tggacagctt caaggaggag ctggacaagt acttcaagaa tcacaccagc cccgacgtgg 
     4321 acctgggcga catcagcggc atcaacgcca gcgtggtgaa catccagaag gagatcgatc 
     4381 ggctgaacga ggtggccaag aacctgaacg agagcctgat cgacctgcag gagctgggca 
     4441 agtacgagca gtacatcaag tggccctggt acatctggct gggcttcatc gccggcctga 
     4501 tcgccatcgt gatggtgacc atcatgctgt gctgcatgac cagctgctgc agctgcctga 
     4561 agggctgttg cagctgcggc agctgctgca agttcgacga ggacgacagc gagcccgtgc 
     4621 tgaagggcgt gaagctgcac tacacctgat aataggctgg agcctcggtg gcctagcttc 
     4681 ttgccccttg ggcctccccc cagcccctcc tccccttcct gcacccgtac ccccgtggtc 
     4741 tttgaataaa gtctgagtgg gcggcaagct tgatatcgaa ttcctgcagc ccgggggatc 
     4801 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag 
     4861 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     4921 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     4981 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     5041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     5101 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     5161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     5221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     5281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     5341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     5401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     5461 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     5521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     5581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     5641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     5701 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 
     5761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     5821 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     5881 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     5941 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     6001 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     6061 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     6121 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     6181 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     6241 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     6301 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     6361 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     6421 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     6481 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     6541 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     6601 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     6661 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     6721 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     6781 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     6841 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     6901 gaatactcat 
//
